How do neural stem cells go through developmental transitions to generate neurons and glia, the major functional units of the brain? We are currently focused on cellular and molecular mechanisms that guide transitions during the development of the cerebral cortex, the highest processing center of the brain. Our lab has been studying important mechanisms that regulate the proliferation of progenitors and migration of their progeny in the embryonic cerebral cortex and postnatal olfactory bulbs. We have identified the zinc finger transcription factor Specificity Protein 2 (Sp2) as a potent regulator of cell cycle progression and differentiation in both the cortex and olfactory bulbs (Liang et al., Development, 2013). Our studies employ genetic mice to conditionally ablate various Sp factors in a cell-specific manner and have yielded novel mechanisms that regulate cell cycle progression and couple it to mechanisms of cytokinesis, cell adhesion, and other exciting cell biological phenomena. The intersection of these cell biological events are critical for transition of stem cells from a proliferative mode of division (symmetric) to neurogenic differentiative (asymmetric) divisions. The lab is hard at work in determining how and to what extent Sp family members cooperate to accomplish this critical developmental transition. (Supported by NIH R01 NS089795)
- Dwyer N.D.*, Chen B., Chou S.J., Hippenmeyer S., Nguyen L., Ghashghaei H.T.* (2016) Neural Stem Cells to Cerebral Cortex: Emerging Mechanisms Regulating Progenitor Behavior and Productivity. J. Neurosci. .
- Loziuk P., Meier F., JohnsonC., Ghashghaei H.T.*, Muddiman DD*. (2016) TransOmic Analysis in Forebrain Sections of Sp2 Conditional Knockout Embryonic Mice Using IR-MALDESI Imaging and LC-MS-based Proteomics. Anal Bioanal Chem. 408(13):3453-74.
- Murlidharan G., Corriher T., Ghashghaei H.T., Asokan A. (2015) Unique glycan signatures regulate adeno-associated viral tropism in the developing brain. Journal of Virology, 89(7):3976-87.
- Liang H., Xiao G., Yin H., Hippenmeyer S, Horowitz J.M., Ghashghaei H.T.* (2013) Neural development is dependent on the function of specificity protein 2 in cell cycle progression. Development. 140(3):552-61.
- Liang H., Hippenmeyer S., Ghashghaei H.T.* (2012) A Nestin-cre transgenic mouse is insufficient for recombination in early embryonic neural progenitors. Bio. Open. 15;1(12):1200-3. COVER ILLUSTRATION
- Ghashghaei H.T., Weimer J.M., Schmid R.S., Yokota Y., McCarthy K.D., Popko B., Anton E.S. (2007) Reinduction of ErbB2 in astrocytes promotes radial glial progenitor identity in the adult cerebral cortex. Genes & Development 21(24):3258-71. MUST READ FACULTY OF 1000
- Ghashghaei H.T., Lai C., Anton E.S. (2007) Neuronal migration in the adult brain: are we there yet? Nature Reviews Neuroscience.8(2):141-151.
- Ghashghaei H.T., Weber J., Pevny L., Schmid R., Schwab M.H., Lloyd K.C., Eisenstat D.D., Lai C., Anton E.S. (2006) The role of neuregulin-ErbB4 interactions on the proliferation and organization of cells in the subventricular zone. Proceeding of the National Academy of Sciences 103(6): 1930-1935.
- Ghashghaei H.T., Anton E.S., Weber J.L., McCann C., Fischer T.M., Cheung I.D., Gassmann M., Messing A., Klein R., Schwab M.H., Lloyd K.C.K., Lai C. (2004) Receptor tyrosine kinase ErbB4 modulates neuroblast migration and placement in the adult forebrain. Nature Neuroscience 7(12): 1319-1328.